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Homozygous male RAGE null (RAGE-/) mice (backcrossed >10 generations into C57BL/6) were generated as described previously [9].
Two independent transgenic lines were generated and backcrossed for >10 generations into a C57Bl6 background.
Mutant mice were generated by standard blastocyst injection and backcrossed for six generations into the C57BL6 background.
SirT1STOP animals were backcrossed two generations into C57BL/6 mice before crossing to APCmin/+ to generate SirT1STOP; APCmin/+ double transgenics.
p19Arf−/− mice were backcrossed 8 generations into C57BL/6 and intercrossed with RIP-Tag2 mice (C57BL/6) to generate RIP-Tag2; Arf+/+, Arf+/−, and Arf−/− mice.
p53+/- heterozygotes [ 26] that were backcrossed more than 10 generations into the FVB/N strain [ 27] were used to generate p53-/ donors.
Arguments like this turn future generations into mere pawns in the power games of the present.
The challenge is to bring younger generations into this shrinking but strongly committed cohort.
The zodiac heads were among the spoils, which disappeared for generations into European art collections.
Three generations into the modern welfare state, and western democracies look less Stalinist than ever.
"Not so now... DNA evidence [can] reopen a family succession many generations into the past.
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