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Vida3 expression in EVida3 has been stable for over 40 generations (data not shown).
Haplotype analysis of a large panel of microsatellite markers confirmed the congenic status of mice after 14 generations (data not shown).
After generation of transgenic mice, the repeat size remained stable for multiple generations (data not shown).
Preliminary analyses determined that stationarity of parameters (alpha, ln likelihood, Fst) was achieved by 25,000 generations (data not shown).
qRT-PCR analysis of some stem cell genes (CD44, Oct3/4 and Nanog) indicated maintenance of their expression along at least eight generations (data not shown).
In contrast, if the above assay was done on SC-Ura, there was no observation of telomeric rearrangement even after 50 or more generations (data not shown).
Similar(49)
The superoxide concentration was proportional to tellurite concentration confirming that K2TeO3 reduction involves O2 − generation (data not shown).
Significant differences in activity of phosphorylated and unphosphorylated WT-PykA and PykA-S37A were not observed in terms of ATP generation (data not shown).
The high number of SNP and phase variation detected in the descendants of Z5463 (calculated mutation rate of about 10−7 per generation, data not shown) even with few generations is in accordance with its hypermutator phenotype due to a non-functional MutS protein [55].
The presence of TRAIL had no effect on withanolide-induced ROS generation (data not shown).
However, a subset of tumours showed these features already in the first or second generation (data not shown).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com