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"By confounding and transgressing the natural boundaries between generations, cloning could strain the social ties between them," it said.
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Assuming that each culture, excluding the parent, began with a single parasite and that populations expanded approximately fivefold per generation, we deduced that the first transcriptional switch most likely occurred, on average, two or three generations after cloning.
One potential approach for distinguishing homologous, homeologous (and paralagous) sequence variation in EST-derived sequences from outbreeders is the generation, cloning and sequencing of PCR amplicons based on the ESTs.
Some critics fear that cloning could usher in a new eugenics, in which nations or individuals might try to improve the average capabilities of the next generation by cloning the likes of an Einstein, Mozart, Michael Jordan or Marilyn Monroe.
The scientists are involved in building the next generation of cloning methods and creating computer code to help them fold DNA into more complex shapes.
The males carrying a translocation and a hemiclone originally sampled from LHM were crossed each generation to "clone generator females".
This plant, a second-generation clone of the original, is placed with its cohort in a polyethylene tunnel, where it is checked weekly for any signs of disease.
Among the eight individuals in the last generation, clones were absent in 43% of the simulations (Figure 2b).
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A second generation of independent clones was sequenced and the distribution of sequences among the first generation clones was compared to that of the second generation.
The global transcriptional profiles of these early generation clones have previously been established during multiple array experiments and the results have been confirmed using northern blot and RT-PCR [26].
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