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Young adult animals at each generation were collected for various analyses (Fig. 1a).
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Surgical, biopsy or ascites HGSOC samples used for PDX generation were collected from chemotherapy-naive patients who underwent surgery or patients treated with multiple lines of prior therapy.
Data for item generation were collected from a postal survey and individual interviews.
First, a subset of embryos from the 28th generation were collected and cultured in normoxia for five consecutive generations.
To test the hypothesis that AF flies are a result of selection of favorable genetic allelic variants, a subset of embryos obtained from the AF flies at the 18th generation were collected and cultured under normoxic condition for several consecutive generations.
Females emerging from these pupae (the F1 hybrid generation) were collected and individually paired with a male from the asexual strain to produce the offspring for the next generation (for further details, see Ma et al., 2013).
In the next generation, males were collected for PCR.
Following the emergence of the next generation, adult flies were collected for seven days and then used to found a new cage for the next generation.
Here, the burn-in was set to 100,000 generations and data were collected for 1,000,000 additional steps.
After standardization, eggs were collected for the generation of the experimental flies at a density of 130 150 per vial (8-10 ml of cornmeal food).
For this study, young adult female mites were collected for gene-expression analysis 35 generations after the initial host shift.
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