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We used the breeding population pedigree information (relationships between first and second generation) to detect possible Mendelian Inconsistencies (MIs) between parents and offspring using the PedCheck software [37].
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In the case of IS4 and IS150, the elements were present in the ancestral genome as a single copy; thus, even if they were to transpose at a rate as high as 10−5, for example, we would need more than 100 000 generations to detect one event (a timescale larger than in our experiment).
Indeed, many researchers have recently used next generation sequencing to detect SNPs in a variety of organisms [ 5- 9].
Testing for HCV relied on a third generation ELISA to detect anti-HCV antibodies and confirmation or genotyping with PCR is currently not available in most centres.
This study, however, suggests a novel quantitative tool for the assessment and comparison of the ability of microarrays and next generation sequencing to detect mRNA transcripts from malignant B-cells in a pool of non-malignant cells.
Our findings indicate that a subset of anti-CCP− patients actually do possess ACPAs, highlighting the need for next-generation diagnostics to detect ACPAs in these patients.
The two primary second-generation strategies to detect SVs involved a "read-pair" (RP) method applied to Illumina paired-end short sequence reads and a complimentary "split-read" (SR) method applied to longer pyrosequencing reads generated by the Roche/454 platform (Stewart et al. 2011).
A modified MoG-based approach by replacing the mean pixel intensity value with the recent pixel intensity value in background frame generation performs better to detect object in a general situation [71] compared to other approaches.
In this study nonlinear wave propagation based on the cumulative second harmonic Lamb wave generation is used to detect micro-structural damage and the corresponding material degradation experimentally.
Then, cISI analysis was used on small networks (characterized by few neurons and few connections) to uncover inhibitory connections (which are expected to delay or even to take out spike generation), as well to detect the temporal integration of successive stimulations [21].
This emphasizes the power of the next generation sequencing approach to detect novel transcripts or even novel genes.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com