Every four days, 1/64 of the culture was transferred to fresh medium, which allows for an average of 6 generations per cycle.
In our experimental conditions, the low-density populations will go through a few more vegetative generations per cycle than the high-density populations, giving greater selective opportunity to any clone that grows faster.
The deletion strain was grown under optimal laboratory conditions (rich medium at 30°C) and was diluted daily into a fresh medium by a factor of 120, corresponding to approximately 7 generations per cycle.
It should be noted, however, that progeny testing requires an extra generation; hence gain per cycle of selection must be double that of simple phenotypic selection to achieve the same rate of gain per unit time.
Similarly, mean lifetime V k, Ne per generation, and Nb per cycle calculated from the demographic data were within 1% of the expected (AgeNe) values for most species and within 4% for all species (Table 2).
Colonies were initiated from single cells, so the number of generations per growth cycle was estimated as log2 of total cell number.
Every 4 days1500 μL was transferred into 9.45 mL of fresh medium, a 1/64 dilution that permitted an average of six generations of growth per cycle and a final population size of ~1x10.
In this method, thermoelastic damping is interpreted as the generation of thermal energy per cycle of vibration and consequently is expressed in terms of entropy a thermodynamic parameter measuring the irreversibility in heat conduction.
Instead of expressing TED in terms of a commonly used complex frequency value, this work calculates TED by using a thermal-energy approach in which the generation of thermal energy per cycle of vibration is considered.
We were first interested in evaluating the general impact of two biases we encountered in the context of analyzing next-generation sequencing datasets: nucleotide per cycle bias and mappability bias.
Observations show a short cycle with almost one generation per year, and a slower life cycle with one generation every 2 or 3 years (Lempérière 1994).
