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The routine generation of transgenic plants involves analysis of transgene integration into the host genome by means of Southern blotting.
Both systems generally offer several advantages over the generation of transgenic plants, including production speed and high expression yields.
Generation of transgenic strains: Transgenic animals were generated as previously described (Hunt-Newbury et al., 2007).
Generation of transgenic animals allowed us to further study the expression pattern of the transgene.
CRISPR Cas9 has revolutionized the generation of transgenic animals.
The generation of transgenic pigs as potential organ donors for humans.
Krimpenfort, P. et al. Generation of transgenic dairy-cattle using invitro embryo production.
Whitelaw, C. B. et al. Efficient generation of transgenic pigs using equine infectious anaemia virus (EIAV) derived vector.
Lu, C. & Kang, J. Generation of transgenic plants of a potential oilseed crop Camelina sativa by Agrobacterium-mediated transformation.
Dr. Mesaeli's lab uses Molecular and Cellular Biology techniques in addition to generation of transgenic mouse models in their studies.
Finally, new gene targeting tools are in development that may substantially simplify generation of transgenic animals.
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