The reduction of Rp led to the generation of three subpopulations of cells in proliferative, quiescent and differentiated states.
However, no significant differences were found regarding agonist-induced cAMP generation of T-cell subpopulations in patients with RA compared with HDs (data not shown).
It has been suggested that the generation of the different subpopulations is linked to regional differences, defined by the expression of particular combinations of transcription factors, in the specification of progenitor cells in the subpallium (Wonders and Anderson, 2006; Flames et al., 2007; Wonders et al., 2008).
The first generation were exposed to photographs of randomly assigned "seed" towers, and later generations were exposed to photographs of the towers built by the members of the previous generation of their own subpopulation.
To first understand the generation of the major subpopulation of CD4+ThPOK− T cells in 4in8 mice, we considered that the transient downregulation of Cd8-encoded CD4 proteins during positive selection would disrupt, albeit transiently, MHCII-specific TCR signalling in CD4-dependent thymocytes (Sarafova et al, 2005, 2009).
The recent generation of PGRST2 and PGRST3 subpopulations from the predominant PGRST1 accommodates either the double-strand break repair (DSBR) or the synthesis-dependent strand annealing models [ 41, 42] as the molecular basis for this gene conversion event.
Neutral fluorescent markers, for example, have been used to monitor the contractions and expansions of subpopulations over 500 generations of evolution (Kao and Sherlock 2008).
The default scenario was based on a constant size population of N = 600 run until mean heterozygosity reached approximately 0.8 (10 generations) split into a number n of subpopulations (n = 1, 2, 3, 6, 12) without any migration.
When we considered pre-migration extinction, in the form of absence of at least one breeding pair (i.e. 1 male+1 female) in a subpopulation, there was no difference in number of extinctions per generation between the pinned and control metapopulations (F1,6 = .009, p<0.93), indicating that pinning did not affect the persistence of subpopulations.
We then studied the effects of pinning different numbers of subpopulations (1, 2, 3, 4, 5, 7, 9), pinning strengths (0, 2, 4, 8, 12, 16 individuals per generation) and migration rates (10 %, 20 %, 30 %, 40on the metapopulation dynamics.
