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Generation of random sequences was done by the random generator tool available at the Regulatory Sequence Analysis Tools web page [ 48].
Accordingly, the generation of random sequences has been investigated for a long time.
Six domains of each trial will be assessed: generation of random sequences, allocation concealment, blinding, incomplete outcome data, selective reporting and other sources of bias.
The quality of the RCT studies was assessed using a modified Jadad scale [ 11], in which the generation of random sequences, blinding method, and reasons for withdrawal and dropout at the time of follow-up were evaluated.
Furthermore, generation of random sequences (TTS and no-TTS) showed that no-TTS random sequences have a much larger probability to be transcription factor binding sites than TTS random sequences.
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We assessed the generation of random sequence, concealment of allocation, blinding of allocation and incomplete outcome data [ 23].
The scale is a score from 0 to 7 (highest level of quality) according to generation of random sequence, allocation concealment, blinding, and withdrawals of clinical trials.
Efficacy of adjunctive CLB in refractory focal epilepsy was assessed only in a small double-blind, randomized, placebo-controlled, cross-over study hampered by several methodological limitations (unclear allocation concealment, generation of random sequence and method of blinding) [ 14].
Berger [ 13] has added to the debate by presenting the logical consideration that successful control for bias cannot be assured on a basis of inclusion of adequate methods, (adequate generation of random sequence, allocation concealment, blinding/masking) alone.
The tool contained the following items: generation of random sequence, allocation concealment, blinding, incomplete data reporting, selective reporting results and other problems that could put the study at a risk of bias.
When the methods in some studies were not described clearly such as the generation of random sequence, we tried to contact the first or corresponding author to get additional information by letters or e-mails.
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