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This technique uses heat shock to induce FLP recombinase, which induces the generation of random clones of GAL4 expression cells at precisely defined time point for cell growth analysis.
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Supported by mathematical calculations, we designed a high-density mutagenesis procedure for the generation of a mutant library of which a limited number of random clones would suffice to exactly localize amino acid residues essential for a particular protein-protein interaction.
The EST generation process involves sequencing of single segments either 5′ end or 3′ end of random clones from cDNA library of an organism.
NotI digestion of random clones indicates an average insert size of 102 kb.
A number of random clones (E1-E16) from last phage pool were tested in ELISA and sequenced.
For large-scale DNA sequencing (EST generation), random clones were grown in antibiotic selective medium for 22 h and plasmid DNA was isolated using alkaline lysis [ 46].
For the generation of random numbers, the 'MATLAB5 generator' was used.
Generation of random oligonucleotides has been refined and various strategies for random oligonucleotide design were developed.
Generation of random network is described in the methods section.
It allows perpetuation of the label along with generation of random combinations.
A total of 68 random clones were selected and sequenced.
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