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The early generation of markers was directed primarily at the identification of adenocarcinoma, negative staining of both malignant mesothelioma and reactive mesothelial cells being accepted by exclusion.
This novel tool uses a new generation of markers called Single Position Polymorphisms or SPPs [ 12].
For generation of markers derived from NBS encoding sequences we also used a modified AFLP (amplified fragment length polymorphism) technique known as NBS profiling.
However, the major drawback of the traditional approaches used to develop microsatellites is that the de novo generation of markers is highly time-consuming and/or expensive due to the requirement to enrich genomic DNA for repeated motifs, cloning, and sequencing.
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This segment of the code stream is only parsed by the program code responsible for the FLEXBITS; thus, possible generation of marker bits poses no problem.
We have shown that GBS is an effective approach for the generation of marker-dense genetic maps in cultivated barley.
The high quality marker data helped in generation of marker linkage groups without obvious inflation of map distances that can be caused by false linkages [ 45].
The development of microsatellite markers will have a major impact on genetic analysis and breeding of melon, especially on the generation of marker saturated genetic maps and implementation of marker assisted breeding programs.
The automated bioinformatic pipeline described here allows the large-scale generation of marker candidates useful for map construction and comparisons in legumes and grasses and, by extension, to any phylogenetic clade with appropriate comparative sequence information.
The last decade has shown the emergence of new generations of markers which allow such a search [ 14].
After four generations of marker-assisted backcrossing, the BC4F3 and BC4F4 lines were designated as NILs.
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