Sentence examples for generation of expression from inspiring English sources

GATEWAY cloning technology (Invitrogen) was chosen for the generation of expression vectors making it possible to rapidly generate vectors containing the same coding sequence with different vector backbone options (for example N or C-terminal tags, different expression vector cassettes).

Yeast combine comparatively low cost and short culture times with straightforward generation of expression clones.

Generation of expression matrices, data annotation, filtering and processing have been previously described [19].

Generation of expression matrices, data annotation, filtering and processing were performed as described previously using the TableButler software package (http://www.OncoExpress.org/software/tablebutler) [25], [26].

Ligation-independent cloning (LIC), sometimes also referred to as ligase-independent cloning, is a simple, rapid and relatively cheap method for the generation of expression constructs.

MIG conducted experiments including design and generation of expression constructs, yeast mating assay, Western blotting and qRT-PCR.

Fundamental to the generation of expression-based diagnostic biomarkers is accurate clustering of patients by diagnosis.

Details of the generation of the expression construct, E. coli expression, protein purification, and enzyme assay for AtAPSK (A. thaliana APSK) were previously described [ 10, 30– 30].

Multiple transcription factors (TFs) are involved in the generation of gene expression patterns, such as tissue-specific gene expression and pleiotropic immune responses.

DNA Constructs: The generation of the expression constructs for human CRMP proteins have been described previously [ 20], while human tau expression constructs were obtained from MRC Protein Phosphorylation Reagents, University of Dundee.

Originally TAS involved the use of two copies of a cell-specific promoter leading to generation of large expression cassettes, which can be hard to use given the space limitations of the conventional viral gene expression vectors.