Sentence examples for generation of construct from inspiring English sources
Insertion of this promoter fragment into XbaI-cut ppcA-S-Fp resulted in the generation of construct ppcA-PRFp-DRFt.
Construction of Transgenes For generation of constructs recombineering was employed (Dolphin and Hope, 2006 ; Tursun et al., 2009 ) and accompanying protocols were modified to construct latrophilin transgenes using cosmids, PCR-amplified targeting cassettes, and positive antibiotic selection.
We have described the generation and utility of construct collections designed to simplify and facilitate the building of fosmid-based FP reporter constructs via counter-selection recombineering.
BAC genomic library clones (rTgV; GenOway, Lyon) were used for the generation of targeting construct to generate heterozygous PrRP- Cre and LSL- PrRP mice.
The 3D printing of this hydrogel resulted in the generation of constructs with tailorable porosity and good handling properties.
The two types of polymer fibre allow the generation of constructs with distinct characteristics in terms of the surface physico-chemistry, mechanical performance and degradation capability, which has an impact on the resulting cell behaviour at the surface of the respective biotextiles.
In this context, what is needed are methods that allow generation of constructs or construct libraries containing enough genes for pathway engineering, i.e. in the size range of 10 to 100 kb.
The generation of constructs, and preparation and purification of proteins were as previously described (64).
The oligonucleotide primers used for the generation of constructs are shown in Additional file 1: Table S2.
With the approach described here, the generation of constructs is straightforward, relying on the use of synthetic oligonucleotides and a single restriction site that ensures directional cloning.
NK conceived, coordinated and carried out molecular generation of constructs (plasmids and BACs) for additive gene transfer and gene targeting experiments and drafted the manuscript.
