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The ratio of experimental to tester individuals after one full generation of competition provided the data for the estimation of a fitness coefficient, which we term population-wide fitness; see data in [ 30].
The mean number of GFP/wild type L1s scored per population sample after one generation of competition was of 330/902 with the 2.5% and 97.5% quantiles being of 92/243 and 1,031/2,618 individuals, respectively.
For several α values we obtained the sex ratios after one generation of competition and estimated allele frequencies assuming random mating and selfing and no sex ratio segregation distortion.
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Total number of insects, mortality by insecticide, insect body mass and respiration rate were recorded after three generations of competition.
The first metric of this effect is the impact of the competitor on the focal species' population growth rate (dN/dt) in the first 20 generations of competition.
In the first, I examined the effect of the competitor on the focal species' dN/dt over the first 20 generations of competition, i.e., up through generation 40.
Because very few competitor wins were recorded at the rates of change examined in the first simulations (i.e., during the first 20 generations of competition), I extended the dE/dt landscape an order of magnitude slower (see Methods).
The size of the competitor's gene network and the rate of recombination, conditional on interactions with the rate of environmental change (dE/dt), accounted for 79% of the variance in the focal species' dN/dt during the first 20 generations of competition (Table 1).
In the absence of viral infection, we could not detect in standard laboratory conditions over 10 generations of competition any positive or negative effect of the drh-1 deletion and the introgressed surrounding region.
The slope of the regression line appears to be insensitive to starting frequency and is weakly affected by the number of generations of competition: So long as the competition experiment proceeds for four or more generations, s l exceeds s d by a factor of 1.7.
To rule out the possibility that cells with SUL1 amplification were selected during the 15 generations of competition used to determine the relative fitness, we performed qPCR on a sample from the last day of the competition experiment in which 96% of the cells corresponded to the Population 3 clone.
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