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Here, as expected, human SAP also significantly reduced the histopathological extent of bleomycin-induced collagen deposition (Fig. 3 A) and the generation of collagen at the protein (Fig. 3 B) and gene level (Fig. 3 C).
Indeed, osteoblast-derived interstitial collagenase initiates bone resorption by the generation of collagen fragments, which in turn activate bone-resorbing osteoclasts [ 37].
The magnetically induced generation of collagen by chondrocytes was also visualized using fluorescence microscopy, with large amounts of extracellular collagen I and collagen II visible in samples exposed to an AMF.
For the detection of collagenase-induced generation of collagen type 2 neoepitopes in the cartilage matrix, a rabbit antiserum raised against the C1,2C (COL2 3/4Cshort) neoepitope was used (IBEX Technologies, Montreal, QC, Canada).
Results suggest that stem cell-based therapeutic modalities have the potential to decrease fatty infiltration after cuff repair, offer improvement in tendon-to-bone healing, increase generation of collagen I and improve the tendon's mechanical properties.
Functionally, they inhibit collagen type II-specific T cell proliferation and pro-inflammatory Th1 cytokine production, and more importantly, induce the generation of collagen type II-specific CD4+CD25+FOXP3+ Treg cells from highly purified CD4+CD25- T cells in an IDO-dependent manner.
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Their interaction with collagen type IV (CIV) is a triggering step that induces generation of collagen-specific auto-antibodies.
Immunocytochemical staining was also used to evaluate the generation of collagen-II.
No correlation could be found between treatment groups and the generation of collagen-specific antibodies (Additional file 8).
9 Thermal damage, including NIR irradiation, denatures the collagen and encourages the generation of new collagen, resulting in tighter skin.
In the dermis, violet second harmonic generation (SHG) of collagen fibers is observed, as well as blue/green autofluorescence of elastic fibers.
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CEO of Professional Science Editing for Scientists @ prosciediting.com