A triangulation of methods tailor-made for every specific stakeholder group allows the gradual deepening and broadening of participation in problem definition, knowledge generation, development of applicable solutions and implementation.
In this context, microorganisms, their products and their processes are essential resources for a new generation of biotechnologies applicable to plant production and protection, and potentially a paradigm shift in agricultural practice.
Furthermore, we have updated the individual semantic search systems that are queried by PathText 2 and updated the generation of queries applicable to these systems.
Another objective is the generation of a clinically applicable gene signature, and this requires transferring the markers from the microarray platform to a robust PCR-based platform.
We believe that the optimised method for generating stable immortalised cell lines developed in this study will be generally applicable to generation of cell lines from other bat species.
While these methods are generally applicable to the generation of Y2H libraries from any source, including mammalian and plant genomes, the potential of functional clones interacting with host proteins to inhibit growth would make this approach most relevant for the study of prokaryotic genomes.
DNA transposition-based strategies offer substantially more efficient random strategies and provide means to generate single-copy insertions, thus potentiating the generation of genome-wide insertion libraries applicable in genetic screens.
The protocol used in generation of the methionine auxotroph is applicable more generally and, because it introduces a gene deletion using a suicide plasmid, can be applied repeatedly.
Kelm, J. M., Timmins, N. E., Brown, C. J., Fussenegger, M. & Nielsen, L. K. Method for generation of homogeneous multicellular tumor spheroids applicable to a wide variety of cell types.
PRISM (Panchromatic Remote-sensing Instrument for Stereo Mapping); AVNIR-2 (Advanced Visible and Near Infrared Radiometer type 2) and PALSAR (Phased Array type L-band Synthetic Aperture Radar), for the generation of a spatial information infrastructure applicable to the geographic information system (GIS) useful to the various kind of applications.
