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However, the generation of a standard curve for each qPCR experiment set-up can be expensive and time consuming, especially for studies with large numbers of unknown samples.
The analytical sensitivity of the real-time qRT-PCR assay was achieved through the construction of an in-house PPRV cRNA for the generation of a standard curve.
Cyclophilin was used for the generation of a standard curve and normalization of the RNA concentration.
Generation of a standard curve verified linear amplification of eGFP and CDX2 genomic DNA amplicons at similar efficiency (Fig. S1F).
For generation of a standard calibration curve, the SVC21 plasmid containing the full-length HIV-1HXB2 viral DNA was used as a template.
Due to the absence of a recombinant sFcεRI protein for the generation of a standard curve, the specific, blanked OD was used for semi-quantitative analysis of serum sFcεRI levels.
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We therefore suggest that the generation of a reliable standard curve for HPV gene quantification requires averaging of replicate calibration runs, rather than depending on a single run.
The use of this bacterium for the generation of a universal standard curve for the assessment of total microbial counts was already described by Kirakodu et al. [ 20].
Bioclipse supports both the generation of standard and non-standard InChIs, and a preference allows for selecting between the different versions.
The generation of a plasma DNA standard curve was accomplished using human genomic DNA.
The calculation of the proportion of expected infections produced by secondary cases at the time when their corresponding index case is reported (PIR2) is conducted in the same way as that of PIR1, but a 2-generation interval is used instead of a standard generation interval.
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