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This consisted of generation of a cell line expressing red-emitting fluorescent protein under the control of a p53 consensus promoter (p53DsRed) [21].
Cloning of transgenic BY-2 cells: generation of a cell line with strongly reduced heterogeneity.
In conclusion, the work presented herein describes the first example of the generation of a cell line that is resistant to APL, which is currently undergoing clinical trials.
For the generation of a cell line stably expressing Syb2-HA, human Syb2 coding sequence in fusion with HA was cut from pCI neo Syb2-HA and cloned into pBABE puro.
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Here, we have developed a protocol for the efficient generation of a cell-laden and orthogonal-multilayer tissue-engineered (TE) corneal stroma, which is induced by the mechanical effects of compressed collagen (CC) or stretched compressed collagen (SCC).
This bacterium has been extensively studied due to its finely regulated asymmetric cell division, which results in the generation of a stalked cell and a motile cell [ 24].
Generation of a new cell layer requires cells in the original layer to divide periclinally only one time.
Using a cell permeable Cat inhibitor, we show Cats in influenza-exposed DCs to be functional and required for generation of a T cell epitope from intact ovalbumin.
This task is complicated by the fact that some of these regulators are involved in the differentiation of multiple cell lines, and that some of them also have multiple roles in the generation of a single cell type.
The chemokine gradient attracts the cells towards the highest concentration, modifying their random movement accordingly, and induces the generation of a new cell in every square where the chemokine concentration is above a certain threshold (CT).
In this hierarchical concept, transition through defined intermediate stages of decreasing potentiality is regarded as prerequisite for the generation of a somatic cell type.
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CEO of Professional Science Editing for Scientists @ prosciediting.com