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With the aim of evaluating the performance of latest generation kits in real forensic caseworks the authors retested some bone samples previously analyzed in their laboratory over the past five years with the old commercial kits.
In conclusion, the authors can claim that the latest generation kits have proved to be decisive in all cases, including those where the previous use of traditional kits did not produce reliable and uniquely interpretable results.
Newer generation kits decrease rates of false positive testing[ 21].
It would be very dangerous to use elaborated stimulation protocol relaying on the results from the II generation kits.
In the present study we aimed to asses if the new generation kits share the same accurate correlations.
The difference between both 2nd generation kits was 40%, which contradicts the manufacturer's information about the identity of both assays.
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Diluted cDNA (10 nmol/L) was amplified, sequenced, and single-read clusters generated using cBot Cluster Generation Kit and TruSeq SBS Kit v5 (36 cycles).
A HiSeq Paired End Cluster Generation Kit was used to generate the clusters and a TruSeq SBS Kit v3 was used for sequencing.
In brief, fragmented cDNA was fixed to a flow cell, and clusters were generated using a Paired-End Cluster Generation Kit v4 (Illumina).
A single read cluster generation kit v.2 (Illumina) was used to generate the clusters and a single read sequencing kit v.3 (Illumina) was used for sequencing.
An approximately 300-bp length cDNA library was prepared using a genomic DNA sample prep kit (Illumina, San Diego, CA, USA), and DNA clusters were generated on a slide using a Cluster Generation kit (ver. 2) on an Illumina cluster station (Illumina), according to the manufacturer's instructions.
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