Diluted cDNA (10 nmol/L) was amplified, sequenced, and single-read clusters generated using cBot Cluster Generation Kit and TruSeq SBS Kit v5 (36 cycles).
A HiSeq Paired End Cluster Generation Kit was used to generate the clusters and a TruSeq SBS Kit v3 was used for sequencing.
In brief, fragmented cDNA was fixed to a flow cell, and clusters were generated using a Paired-End Cluster Generation Kit v4 (Illumina).
A single read cluster generation kit v.2 (Illumina) was used to generate the clusters and a single read sequencing kit v.3 (Illumina) was used for sequencing.
An approximately 300-bp length cDNA library was prepared using a genomic DNA sample prep kit (Illumina, San Diego, CA, USA), and DNA clusters were generated on a slide using a Cluster Generation kit (ver. 2) on an Illumina cluster station (Illumina), according to the manufacturer's instructions.
Clusters were generated using Illumina's Single Read Cluster Generation Kit v4 (GD-103-4001) or Paired End Cluster Generation Kit v4 (PE-203-4001), respectively.
Each of the quantified sample libraries were prepared for sequencing utilizing a paired-end cluster generation kit (v4), and Illumina's cBot instrument to generate clusters.
Clusters were generated in a cBot Cluster Generation System using the Paired-End Cluster Generation Kit v2-HS and sequenced on the Illumina HiSeq 2000 platform (Illumina) with a 2x50 base-pairs (BP) paired-end mode.
Sequencing was performed in a pool of equimolar amounts of each library on one lane of a 75-cycle double indexed paired-end Illumina Genome Analyzer IIx run (sequencing chemistry kit v4, cluster generation kit v4, recipe version v7.4) (Kircher et al. 2012) and generating 1,021,385 sequencing reads for the tooth and 623,912 for the rib.
Five picomoles of DNA from each strain were loaded onto two lanes of the sequencing chip, and the clusters were generated on the cluster generation station of the GAIIx using the Illumina cluster generation kit.
A passengers car SI engine equipped with a "third generation" kit for the dual-fuel operation was tested using a dynamometer test rig.
