Because weedy rice is a predominantly self-pollinating taxon, F2 and F3 generations generated by selfing will be much more common than backcrossed generations.
A three-generation design was generated by-intercrossing two experimental meat-type chicken lines that were divergently selected on fatness [ 39], referred to as fat (FL) and lean (LL) lines.
After harvesting the first generation, the MIC was tested and a second generation was generated by exposing the first generation at its sub-MIC (66%).
To validate the association of other three novel Pi2/9 homologues with the blast resistance, monogenic lines at BC3F3 generation were generated by marker assisted backcrossing (MABC).
In this case, the scores were determined in the order of A, D, C, B. According to the ranking conditions, G-Types in the next generation were generated by the selection methods in genetic operations as shown in Table 4.
Each generation was generated by factorial mating of 20 males and 500 females, with four half-sib progenies per female.
The technique is based on the attenuation, by the patient's body, of a generation beam generated by an X-ray source with two energy levels.
Each generation is generated by multinomial sampling, where the probability of choosing an allele of a given type (ancestral or derived) is weighted by its respective (marginal) fitness.
Homozygous IVA-PLA2-knockout mice with a C57BL/6 background (more than N19 generations) were generated by the cross breeding of heterozygous IVA-PLA2-knockout mice, which were a generous gift from Dr. T. Shimizu and Dr. N. Uozumi (Department of Biochemistry and Molecular Biology, the University of Tokyo) [31].
Subsequent generations were generated by selecting 300 males and 300 females among the selection candidates on their estimated breeding values and performing random mating to form 300 full-sib families in each generation, assuming non-overlapping generations.
