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At BC3F1 generation, a total of 120 alleles from 60 markers were observed.
Every generation, a total of 15 25 female offspring from wasps of the short-memory retention lines were selected to continue independent introgression lines.
At the F6 generation, a total of 1615 chicks were produced (98 to 248 per family) and phenotyped for MD resistance measured as survival time in days after challenge with a very virulent plus (vv+) strain of MDV.
After rescreening and phenotyping of the BC1F2 generation a total of 61 confirmed recombinants were retained for the informative recombinant mapping subset, each containing a single recombination event within the map interval.
In the F5 generation, a total of about 30 males and 120 females per FSIL-family were chosen and mated at random to produce the five independent F6 replicate FSIL-families, which constituted the mapping population.
For example, for scenarios with 500 QTNe in a given generation, a total of 1251.7 distinct QTNe were observed across the 20 generations for scenario T5se but only 314.6 distinct QTNe for scenario A25se.>> When generation 0 was assumed to be the base population, the amount of genic variance that the QTNe explained in a given scenario closely matched the number of QTNe in that scenario (Table 5).
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Bayesian phylogenetic trees were obtained employing MrBayes Version 3.1.1 [ 141]; four chains were run simultaneously for 2000000 generations and trees were sampled every 100 generations generating a total of 20000 trees.
Bayesian inference trees were obtained employing MrBayes version 3.1.2 [ 49]; four chains were run simultaneously for 4 × 106 generations and trees were sampled every 100 generations generating a total of 40000 trees.
Across generations, a total of 512 males were mated in a nested mating design to 941 females to produce 81,429 tagged progenies.
The program was run for 150,000 generations, a total of 1,501 trees were sampled and after burning-in the trees 376 1501 were evaluated.
In a single next-generation run, we generated a total of 319,361 sequences: 251,032 derived from tissues plus another 68,329 derived from the unselected CX7C library before administration.
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