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All the rules governing management of the cosmos are engraved on the shaft of Wotan's mighty spear, fashioned from the tree that cost him his other peeper, thereby generating the expression "turning a blind eye".
The maqu_2220 fragment in pXT71 was digested with NdeI/ XhoI (Takara Co., Ltd ,Kyoto, Japan) and ligated to the same site of pXT37b [ 15] generating the expression vector pFQ52.
The model variant ICMg2 has three additional hyperparameters for generating the expression data, which we set to μ0 = 0, = 1 and σ2 = 0.1 to describe small variations around the base value of zero.
CFL carried out the subtraction hybridization, the initial experiments cloning the genes, generating the expression and siRNA constructs, and drafting the manuscript, JRM carried out the analysis of the genes in different species, and assisted in drafting the manuscript.
A major focus of this study was elucidation of how important different effector pathways downstream of RAS are in generating the expression profiles exhibited by certain cancer cell lines.
It is applied to the pairs of expression profiles in ℒ and aims at maximizing the model likelihood: (1) with P g, g' |Θ) being the probability of generating the expression profiles associated with genes g and g' with the HMM with parameters Θ.
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It remains unclear whether these regions make different contributions to a single aversive learning process or represent independent learning mechanisms that interact to generate the expression of pain-related behavior.
Then, pBBR1MCS-2::p lac ::phaC1AB was digested with SspI to generate a 4.7-kbp expression cassette of the phaC1, phaA and phaB1 under control of the lac promoter and ligated with the EcoICRI linearized fragment of pBBR1MCS-2::aldhaTdhaT::pct (Heinrich et al., 2013) to generate the expression vector pBBR1MCS-2::p lac ::aldD::dhaT::pct::p lac ::phaC1AB.
To demonstrate that the 2A site is functional in our construct, the pEnt-Emr/Tet cassette was recombined by the Gateway method into the pDest27 expression vector to generate the expression vector pExp/pD27/pEnt-Emr/Tet (Fig. S2).
Sequencing a 200-bp fragment of the phoP gene in the H37Ra and H37Rv strains utilized in our laboratory, confirmed this SNP to be present in the H37Ra used to generate the expression data presented here (data not shown).
Furthermore, plasmid pRARE was isolated from Rosetta (DE3) pRARE cells and used to transform E. coli BL21 Star (DE3) (Invitrogen) to generate the expression strain BL21 Star (DE3) pRARE.
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