This can be overcome by generating suspensions of enzymatically dispersed whole single cells which can then be isolated manually (10), or by FACS or microfluidic approaches (43, 63, 76– 76).
However, a skeptic seems to mechanically apply the skeptical Modes, in order to generate suspension of judgment and tranquility.
Two explants were used to generate suspension cultures: stably transformed explants were placed on MS medium supplemented with 0.5 mg/l 2,4D (2,4-dichlorophenoxyacetic acid) and 40 mg/l kanamycin for the induction of callus, which was transferred into liquid MS medium supplemented with 1 mg/l 2,4D and 50 mg/l kanamycin.
In some experiments, caspofungin was used in combination with 2 μg/ml nikkomycin Z. A. fumigatus spores were collected and serially diluted to generate suspensions containing 1 × 10 to 1,000 spores/ml in sterile water.
The reduction of transition metal salts in solution is the most widely used method for generating colloidal suspensions of metals.
The integument calli that developed were transferred to MS medium containing 2 mg/mL of 2, 4-D and 0.1 mg/mL of thidiazuron, pH 5.7 under controlled conditions for generating cell suspensions.
Although we had some difficulty in generating uniform suspensions of this bacterium which interfered with our binding assays, all the trends observed with BCG-lux could be confirmed with Mtb (Fig. 2A E; right panels).
Comparing these data generated under suspension plus FCS with data generated under suspension minus FCS revealed that PINCH1−/− MEF maintain their level of radiation cell survival independent from FCS over a time interval of 7 h while the radiation survival of PINCH1fl/fl MEF showed a reduction with prolonged FCS withdrawal (Fig. 2C).
Mammospheres were generated in suspension culture from six DCIS samples.
Organ tissues were weighed and then pulverized to generate a suspension of tissue in TSB.
