Sentence examples for generating stranded from inspiring English sources

With the advent of many strand specific RNA library preparation protocols increasing number of RNA sequencing experiments are generating stranded RNA sequencing data [ 7– 9].

The, non-stranded, Illumina TruSeq kit can be adapted to generate strand specific libraries and can be used to access detailed information on the transcriptome.

We have modified an existing non-stranded automated RNA library protocol into a protocol that generates strand specific libraries by using the Illumina TruSeq kit in combination with other reagents.

We generated strand specific cDNA probes in situ after covalently binding NH2-modified cDNA inserts onto cross-linked N-hydroxysuccinamide slides in a strand specific manner.

However, consistent with the findings of Haddad et al. [ 70] our extensive attempts to generate strand specific amplicons were unsuccessful (data not shown), therefore a microarray approach was employed.

Further we show that replication plays a key role in generating strand asymmetry, and that the relative contribution of selection and mutation varies among nucleotides.

It inactivates virus infectivity by generating strand-breaks in the genetic material and has the further advantage, compared with chemical agents, of high penetration into and through biological materials [7].

One of the most reliable design strategies for generating strand-biased siRNAs is to use asymmetric 25/27-nucleotide 25/27-nucleotide].

To determine the role of the alignment method in generating strand bias, we aligned our samples using an additional aligner: Bowtie [ 13].

The resulting library was sequenced by Fasteris SA (Switzerland) using the HiSeq 2000 Illumina technology generating strand-specific paired-reads of 2x100 bp.

To generate strand-specific standard curves for ssqPCR, and strand RNA was transcribed in vitro from a plasmid containing a portion of the nsP1 gene from either ONNV or CHIKV.