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Such model calibration allowed determining the diffusion coefficients of silicon vacancies and interstitials at room temperature, the time required for the formation of all the complexes, the concentrations of complexes as a function of H concentration and the specific role of some complexes in generating strain.
Therefore, we continued to insert a terminator (rrnB from plasmid pXMJ19) before argF to reduce the transcription level of argF, generating strain CO-6.
Therefore, the C. crescentus xylD in strain TMB4530 containing xylB, xylD, xylX and xylA was replaced with the counterpart from the halophilic archaeon H. volcanii (HVO_B0038A, xad_Hv), generating strain TMB4531.
This resulting complementation plasmid, designated pDR45, was transformed into S. aureus RN4220 by electroporation and then into KB1045 generating strain KB1046 (Table 1).
Therefore, we introduced the Bacillus subtilis PPTase Sfp, which exhibits a broad substrate specificity [5], in HpPen1, thus generating strain HpPen2 (Fig. 3).
To do this, we transformed A. nidulans strain FGSC A851 with linearized pDLAM002, and selected for growth on medium without arginine (Figure 3A), thereby generating strain DLAN2 (dmtA−/tmdA−).
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We describe the rationale and procedures associated with each step in the context of generating strains possessing enhanced ethanol tolerance.
We reasoned that this would offer a huge potential phenotype space that could be exploited for strain improvement, and specifically for generating strains with altered genetic backgrounds that provide a benefit for heterologous pathways of industrial relevance.
We then performed colocalization experiments by generating strains expressing APL-1 GFP APL-1 GFPy::UNC-108.
cdc24CDK-A/PAK-A was crossed into the cdc42-1, Δbud1, and Δbem1 strains, generating strains RLY3093, 3095, and 3102.
The obtained 1584-bp and 1586-bp PCR products respectively harboring BAT1 or BAT2 sequences were gel-purified and used to transform strain CLA1-2, generating strains CLA31 (bandΔ::kanMX4 BAT2 ura3 leu2::LEU2) and CLA32 (BAT1 bat2Δ::kanMX4 ura3 leu2::LEU2).
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