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The mouse PKCζ RNA was obtained by linearization of the pBluescriptKS plasmid (gift of S. Louvet) by SalI or XbaI (Biolabs) and transcription from respectively the T3 or T7 promoter of the vector generating sense 534b SalI or antisense 540b transcripts.
Total RNA was separated electrophoretically and transferred to a positively charged nylon membrane by capillary transfer [27], UV cross-linked and hybridised with digoxigenin labelled antisense riboprobes of the 3'UTR of CYP3A43 prepared using T7 and T3 RNA polymerases for generating sense and antisense riboprobes respectively.
This can be very helpful in examining the orientation-dependence of a cis-acting sequence or in generating sense and antisense RNA probes.
To validate these target sites, the 3′-UTR of human MXD1 was amplified and inserted in both orientations downstream of the luciferase gene in the pGL3-control vector, generating sense (S) and antisense (AS) constructs, collectively referred to as Luc-1 in Figure 5a.
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It is this more naturally generated sense of style Wasson has made her own.
If the objective of contextualism is to generate sense of place, then it must be judged a failure.
It is as if that quality were kept out frequently, but certainly not always by some strangely generated sense of reserve".
It is as if that quality were kept out — frequently, but certainly not always — by some strangely generated sense of reserve".
The proposed technique uses a feedback loop and a LDO to generate, sense, and regulate the output current.
Artist Matjames Metson creates evocative fine/folk art that is truly American at its soul, the dark, lost, yet still hope-filled side of America that generates sense out of chaos, beauty from despair.
After cleanup, 10 µg cRNA was used to generate sense cDNA.
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