Exact(12)
In its simplest form, competent cells are directly transformed with PCR products from a single amplification step generating overlapping fragments.
Three of these, representing the coding region, were sequenced using primer sets generating overlapping reads on both strands (Table S2).
The process was repeated quite regularly, each after a short period of time, generating overlapping layers.
To confirm this genome sequence, this sequence was re-amplified using 6 sets of PCR primers generating overlapping fragments of the genome which were directly sequenced.
Motifs in the promoter regions of SMU_610, SMU_718, and SMU_1006 were deleted by two initial PCRs generating overlapping DNA sequences.
In addition, MCODE has an optional post-processing step with operations such as filtering non-dense subgraphs and generating overlapping clusters.
Similar(47)
Choosing to generate overlapping read pairs instead of non-overlapping read pairs can reduce the effective coverage by half.
We have generated overlapping pulses to test how robust the proposed system is.
In all cases the aperture and the step size chosen generate overlapping areas in order to increase the resolution of the components [18].
Our approach builds on preparing libraries with tunable size distributions such that sequencing from both ends generates overlapping reads.
This algorithm provides a sequence alignment function for the entire circular genome and designs a set of primers that generates overlapping PCR amplification for the entire circular genome, e.g. the mt genome.
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