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Using one such cell line, K1, we have tested the effect of experimental abrogation of p53 function by generating matched sub-clones stably expressing either a neo control gene, a dominant-negative mutant p53 (143ala) or human papilloma virus protein HPV16 E6.
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A resulting co-precipitated protein of 17 kDa was analyzed by mass spectrometry and peptides generated matched an open reading frame of 525 bp on the genome of B. recurrentis A1 (unpublished data), designated hcpA.
Provide a bit more technical information about the in-house implementation of a molecule fragmentation procedure used to generated matched pairs.
Limitations: One drawback of our method is the need for extant or newly generated matched samples in the source and target platforms.
Variant set enrichment is a permutation-based method that compares the enrichment of genetic risk-variant sets within any functional element (such as H3K4me1-marked putative enhancers) to randomly generated matched genetic risk-variant sets [ 26, 38].
Endothelial-targeted Nox2 transgenic mice (Nox2-Tg), overexpressing human Nox2 under the control of the Tie2 promoter/enhancer, were crossed with ApoE−/− mice (Jackson laboratories, USA) to generate matched litters of Nox2-Tg ApoE−/− and ApoE−/− mice.
We developed an obesity classification scheme by using estabbody mass index (BMI) classification thresholds and prevalences in young adults to generate matching classification thresholds for Fat Mass Index (FMI; fat mass/height2).
We first trained the model using 14 649 protein coding positions for which we generated matching Affymetrix SNP6.0 calls.
Therefore, the raw data from the HILIC LC-FTMS was deconvoluted using Decon Tools, and then, the output of Decon Tools was processed by GlycResoft to generate matching structures and to provide quantitative information.
Instead, the ontology can be used to generate matching pharmacogenomic treatment recommendations for all drugs in the knowledge base, and further filtering or refinement to create targeted clinical decision support messages needs to be done in external applications.
This is ridiculous and wrong: since the justification for the database is fighting crime, access to it should be strictly confined to crime-fighting agencies and its use to generating matches from crime-scenes.
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