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Conservation policies promote their protection but agri-environmental measures for both can be implemented in the same area, possibly generating loss of efficiency.
This pattern is in keeping with the well-documented role of CIN in generating loss of heterozygosity (LOH) and haploinsufficiency of other tumor suppressors (Sotillo et al., 2009).
We have developed several mouse models to investigate the requirement of the DMD for proper imprinting regulation and to determine the role of CTCF binding to the DMD by generating loss of function and gain of function mutations (Fig. 1).
One difference between piggyBac and P element transposons is that the former almost invariably excise precisely, whereas P elements often undergo imprecise excisions that delete flanking sequences – a property that has proved extremely useful for generating loss of function alleles (Voelker et al., 1984; Daniels et al., 1985).
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Betting on old business models and the fossil-fuel generation, they built a massive 80 gigawatts of new fossil power generation capacity in the last 10 years, much of which is already generating losses and now risk becoming stranded assets.
Yet as sales of these have become sluggish, even the television business risks generating losses.
That year, Willow also began generating losses — 9.1 percent, and then 18 percent in 2008 when the credit crisis hit.
G.M.'s venture fund may keep it plugged into the auto tech race, but it will struggle to avoid generating losses along the way.
"Modelzone has been generating losses over the past few years, largely through an increase in online competition and having taken leases on new stores that proved to be unprofitable".
This study provides an easy and effective approach by using the CRISPR/Cas9 system for generating loss-of-function mutants for functional genetic studies of locusts and for managing insect pests.
Thus, our data indicate that experiments with siRNA pools have a greater likelihood of generating loss-of-function phenotypes than individual siRNA duplexes.
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