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After drying, antibiotic discs were placed and incubated at 42°C for 48 hours in anaerobic jar using CO2 generating kits.
The screw caped test-tube was incubated under anaerobic conditions (anaerobic jar supplied with gas generating kits), while the conical flask was continuously shaken at 150 rpm on an orbital shaker.
A total of 32 Lactobacillus isolates, recovered and selected as probiotic candidates in a previous study [ 16], were cultured in MRS broth (Difco) and incubated at 37°C under anaerobic conditions (anaerobic jar supplied with gas generating kits).
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Anaerobiosis was obtained using a gas generating kit.
Promega's expertise in DNA technology has generated kits used in paternity testing and in crime scene investigations, a growing area of its trade.
The cultured plate was incubated in a microbiological culture jar, 10% CO2, 5% Oxygen and 85% Nitrogen, achieved using a Campy-gas generating kit (cat # Oxoid BR0060A) and a palladium catalyst.
The anaerobic conditions were provided by means of a gas generating kit (AnaeroPack, Mitsubishi Gas Chemical, Japan).
The plates, inside jar with the gas generating kit (OXOID), were incubated during 48 hours at 30°C.
The plates were placed in a glass jar, together with a gas generating kit (Oxoid) and incubated at 37°C for 48 hrs.
The enrichment broths were subcultured onto Campylobacter blood-free selective agar (charcoal cefoperazone deoxycholate agar product CM0739, Oxoid Ltd) at 37°C for 48 hours microaerobically, by using a microaerobic gas generating kit (product CN0025, Oxoid, Ltd).
Plates were incubated in a hydrogen enriched microaerophilic atmosphere consisting of 10% CO2, 10% H2, and 80% N2 using Campylobacter system Gas Generating Kit (Oxoid, BR0060A) with palladium catalyst at 37°C for 72 hours, shielded from light.
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