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Anaerobiosis was obtained using a gas generating kit.
The cultured plate was incubated in a microbiological culture jar, 10% CO2, 5% Oxygen and 85% Nitrogen, achieved using a Campy-gas generating kit (cat # Oxoid BR0060A) and a palladium catalyst.
The anaerobic conditions were provided by means of a gas generating kit (AnaeroPack, Mitsubishi Gas Chemical, Japan).
The plates, inside jar with the gas generating kit (OXOID), were incubated during 48 hours at 30°C.
The plates were placed in a glass jar, together with a gas generating kit (Oxoid) and incubated at 37°C for 48 hrs.
The enrichment broths were subcultured onto Campylobacter blood-free selective agar (charcoal cefoperazone deoxycholate agar product CM0739, Oxoid Ltd) at 37°C for 48 hours microaerobically, by using a microaerobic gas generating kit (product CN0025, Oxoid, Ltd).
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Promega's expertise in DNA technology has generated kits used in paternity testing and in crime scene investigations, a growing area of its trade.
After drying, antibiotic discs were placed and incubated at 42°C for 48 hours in anaerobic jar using CO2 generating kits.
A total of 32 Lactobacillus isolates, recovered and selected as probiotic candidates in a previous study [ 16], were cultured in MRS broth (Difco) and incubated at 37°C under anaerobic conditions (anaerobic jar supplied with gas generating kits).
The screw caped test-tube was incubated under anaerobic conditions (anaerobic jar supplied with gas generating kits), while the conical flask was continuously shaken at 150 rpm on an orbital shaker.
Stable cell lines expressing Kit-Fc were generated and Kit-Fc was purified to greater than 95% purity.
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