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The edge diffraction effect is included in the model by generating first order diffraction components for each IS.
With this funding, the company set up its first robotic work cell, built an IP portfolio surrounding the core technologies, and started generating first revenue.
This process occurs in two stages, generating first reversibly "primed" and then irreversibly modified fibroblasts with tumor-promoting properties [1].
Immediately after transcription, external spacer sequences are degraded, generating first 45S and then 41S intermediates.
Reverse transcriptase extends the 3' DNA end of each primer generating first strand cDNA/mRNA hybrid.
RA mechanoreceptors grow central and peripheral axons soon after neurogenesis, generating first order branches (red color).
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β-HMX has a "chair" configuration and is centrosymmetric: forbidden from generating second harmonics.
The complete kit protocol was followed for generating first-strand cDNA pools with intact 5' ends except that both random hexamers and the oligo dT primer provided in the kit were used in a single reverse transcription reaction to obtain full-length cDNA fragments.
After generating first-strand cDNA from total RNA using ThermoScript RT-PCR System (Life Technologies, Tokyo Japan), PCR was performed with AccuPrime™ Pfx DNA Polymerase (Life Technologies) under the following cycling conditions of either 35 (LTR transcripts) or 25 cycles (others): 95 °C for 1 min; 56 °C (β-actin) or 58 °C (others) for 1 min; and 72 °C for 1 min.
Notable aims of the project included developing a model instrument for measuring health literacy and generating first-time data on health literacy across diverse populations in the EU to make a comparative assessment and to provide an empirical basis for European, national and regional health policies.
We used the RNAs for generating first-strand cDNAs using Taqman Reverse Transcription Reagent and the resulting cDNAs for real time PCRs using DPYSL2 transcript-specific primers (Table S5) following manufacturer's protocols (ABI, SYBR Green PCR master mix) on ABI 7900 system.
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