Exact(13)
Four biological replicates, with balanced dye-swap, were used for generating expression profiles.
By contrast, mechanisms generating expression divergence have garnered less attention.
Studies also suggested that chromatin regulators have a key role in generating expression diversity [ 13].
The Arcturus RiboAmp HS method was more reliable at generating expression arrays at the threshold of below a nanogram of total RNA.
Full-length WRKY3 and WRKY4 coding sequences were amplified by PCR with the same gene-specific primers used for generating expression constructs in E. coli as described above.
Furthermore, the MAS5 algorithm for generating expression summaries and the Bioconductor R package were used for the analysis of the publicly available database (see above human hepatocyte studies and [ 13]).
Similar(47)
In our system, this problem arises when generating expressions that refer to an instance.
Amplified PCR fragments were cloned into pET151/D-TOPO to generate expression constructs that express a 6xHis-V5-TEV tagged fusion protein.
In a second experiment, we show that our parametrically generated expression variables correlate with the intended user affect perception.
To this end, we generated expression constructs that produce GFP fusion variants of muSOX and SOX.
For this purpose, we generated expression plasmids for different Flag-Sox2 deletion constructs.
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