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Our results demonstrate the efficiency and cost-effectiveness of next generation sequencing technologies in generating expressed sequences.
These libraries were single-pass sequenced from the 5' end generating expressed sequence tags (ESTs).
We complemented this approach by generating expressed sequence tags (ESTs) from protein-encoding loci of this species.
For aquaculture species, transcriptome analysis of pooled RNA samples from multiple individuals using Illumina sequencing technology is both technically efficient and cost-effective for generating expressed sequences.
We have demonstrated that transcriptome analysis of pooled RNA samples from multiple individuals using Illumina sequencing technology is both technically efficient and cost-effective for generating expressed sequences.
In crop such as chickpea, where limited genomic resources are available, identification of stress-responsive genes can be undertaken by generating expressed sequence tags (ESTs) from stress-challenged tissues.
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By employing site-directed mutagenesis, 4 SULT1allozymesmes were generated, expressed, and purified.
A dimeric immunoenzyme comprising this diabody and Rana pipiens liver ribonuclease I (rapLRI) was generated, expressed as soluble protein in bacteria, and purified to homogeneity.
To delineate the structure-secretion relationship for X12345, blade-deletion mutants of X12345 were generated, expressed in E. coli, and tested for secretion (Fig. 2b).
The Human Genome Project also generated expressed sequence tags (ESTs), which are short double-stranded fragments usually hundreds of bases in length that were derived from tissue-specific cDNA libraries and used for gene discovery.
The neurons generated expressed PSA-NCAM, Dcx and MAP2.
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