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High-quality protein animal feed can be a byproduct of some of the mechanisms for generating ethanol.
The implementation of CBP requires microbes that can produce a functional cellulase system while generating ethanol at high yields and concentrations.
It was observed (Eiadpum et al. 2012) that a co-culture of Kluyveromyces marxianus and Saccharomyces cerevisiae immobilized on thin-shell silk cocoon was effective for ethanol fermentation at a high-temperature, generating ethanol concentrations of 81.4 and 77.3 g L-1 when using temperatures of 37 and 40°C and an initial sugar concentration of 220 g L-1.
In this synthetic control medium, S. cerevisiae 424A completely consumed glucose and xylose in 18 and 72 h, respectively, generating ethanol at a concentration of around 35 g/L (about 80% metabolic yield) and a cell density (OD 600 nm) of approximately 12.
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Yeasts generate ethanol from glucose by alcoholic fermentation under anaerobic conditions.
The yeast (Saccharomyces cerevisiae) and lactic acid bacteria generate ethanol, carbon dioxide and lactic acid as well as other fermentation products like aldehydes, ketones and different organic acids.
Various strains of E. coli have been reported to metabolize glucose (and other sugars) to generate ethanol regardless of culture medium or broth used [3, 27 34].
A constructive development is the use of CO2 (sunlight and water) as a feedstock for the growth of algae using genetically enhanced cyanobacterias to generate ethanol and O2 see Fig. 2.
Aiming to evaluate the potential expansion of the seasonal operation of Louisiana sugar mills (currently operating for only 3 months every year because of the sugarcane availability) as well as to generate ethanol in these mills, Kim and Day [66] studied the utilization of two additional feedstocks: sweet sorghum and energy cane.
Our respiratory-deficient strain can efficiently generate ethanol from aerobic glucose and xylose cultivations.
There are 17 EMs that do not result in biomass production, whereas 9 generate ethanol.
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