Sentence examples for generating disruption from inspiring English sources

Two abortive bomb attempts organised from Yemen last year, both foiled, proved almost as successful in terms of generating disruption and fear as if the bombs had gone off as planned, as AQAP itself boasted.

Open image in new window Fig. 2 Monte Carlo simulation flow chart to generate disruption scenarios.

For reconstituting the generated disruption strains the PMT ORFs plus additional 500 700 bp up- and downstream of the respective START and STOP codons were amplified by PCR with primers containing BamHI restriction sites.

To assess the function of PpHsp16.4 in stress tolerance, we used gene targeting to generate disruption mutants of PpHsp16.4 genes.

In both cases, we again detected ranalexin dependent induction of expression after 15 minute (min) exposure that peaked after 30 min and subsequently declined after 60 min. The Sigma TargeTron system was applied to generate disruption mutants, for which the S. aureus laboratory strain RN4220 was recommended by the manufacturer.

Furthermore, hippocampal lesions generate disruptions both in fimbria/fornix and in white matter integrity of ventral mPFC in monkeys [ 15], referring to the Hip-PFC pathway as the major projection originating in the hippocampus that directly innervates the PFC in primates, as well as in rodents.

For generating PMT disruption constructs PMT open reading frames (ORF) were amplified by PCR from reference strains H99 and JEC21, and fragments obtained were cloned into standard cloning vectors.

These may release an array of proteases that induce the death of endothelial cells, thereby generating local disruption of microvessels [21] and further promoting IPH.

NHEJ is an imperfect repair process that often results in insertions or deletions of DNA bases at the site of the double strand break (DSB) during repair, making NHEJ particularly applicable for generating gene disruptions.

If the efforts at generating mouse disruptions of the lipoic acid synthase gene Lias are in any way instructive to evaluating the chances for success at rapidly generating a FASII knockout mouse, then this does not bode well as these attempts have so far only led to embryonic lethality [34], precluding thorough investigations on live knockouts.

In this study, we demonstrate the successful application of RNA-guided CRISPR Cas9 for generating gene disruptions in CHO-K1 cells.