Cadet, J. & Douki, T. Oxidatively generated damage to DNA by UVA radiation in cells and human skin.
Cadet, J., Douki, T. & Ravanat, J.L. Oxidatively generated damage to the guanine moiety of DNA: mechanistic aspects and formation in cells.
Cadet, J., Douki, T., Ravanat, J. L. & Di Mascio, P. Sensitized formation of oxidatively generated damage to cellular DNA by UVA radiation.
Oxidatively generated damage to RNA, measured as 8-oxo-7,8-dihydroguanosine (8-oxoGuo) in urine, is increased in patients with genetic iron overload, which have led us to test the hypothesis that high iron status, assessed by iron biomarkers and genetic disposition, increases urinary excretion of 8-oxoGuo.
This generated "damage" to the DNA, which would show a positive TUNEL signal and indicate that the TUNEL reaction works.
This may be in part because the idea is difficult to test cleanly, because free radicals not only generate damage to macromolecules, but also affect other processes, such as cellular signalling.
Low concentration of ROS may facilitate signal transduction, enzyme activation and other cellular functions, but high concentration of ROS generates damage to DNA, protein and lipid which can lead to cells transformation such as cancer [ 13, 14].
In this study, we found no significant correlations between ADMA or arginine ADMA and three markers of systemic oxidatively generated damage to DNA (8-oxodG), RNA (8-oxoGuo) and lipids, neither in the individual groups nor in the cohort as a whole.
It is necessary to first generate damage at the element level from the section damage at the fiber level discussed in the previous section.
Our data point towards two ways by which misfolded NTS1 may be generated: Damage by exposure to buffer components and by close contact of the receptor to the neurotensin affinity resin.
