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Primers used for generating constructs are listed in Table 3, and plasmid construction strategies are summarized in Table 4.
Co-cultures of the cell types in multi-compartment hydrogels allowed generating constructs with a layered distribution of collagens and glycosaminoglycans.
Cartilage tissue engineering through the culture of chondrocytes seeded in 3D porous scaffolds has the potential for generating constructs that repair successfully.
The method was optimized by generating constructs for targeting the Leishmania donovani inosine monophosphate dehydrogenase gene (LdIMPDH) encoding six different drug resistance markers, and was found to be rapid and efficient.
We have developed a novel system to study microRNA functions by generating constructs expressing pre-miRNAs and mature miRNAs.
However, we removed the ccdB gene so that all of the multiple cloning sites could be exploited, generating constructs termed "pENTR1A no ccdB" and "pENTR4 no ccdB".
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All generated constructs were sequence verified via Sanger sequencing (Source Bioscience, UK).
The generated constructs were digested by suitable restriction enzymes for gene targeting (Supplementary Fig. 7a d).
The lentivirus was produced by harvesting culture supernatants obtained upon transfecting 293T cells with the generated constructs and packaging plasmids using TransIT-LT1 (Mirus Bio LLC, Madison, WI, USA).
PCR generated constructs have not worked well in our hands for targeting, giving very low percentage, or disappointingly zero targeting.
The set of generated constructs shows which aspects of a particular topic play a role in its perception.
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