The Eco1 acetyltransferase is unnecessary for cohesin's association with chromatin but essential for generating cohesion during DNA replication (Skibbens et al., 1999; Tóth et al., 1999).
Through biased transfers, a group then may be defined by the multiple transfers of the same gene among different closely related taxa, generating cohesion among the recipient organisms in terms of genetic similarity.
Interestingly, the origin recognition complex (ORC) promotes sister chromatid cohesion in yeast (ORC) [67], and it is possible that OriP recruits ORC to generate cohesion between sister viral genomes.
We postulated that this failure to generate cohesion was due to a misregulation of the cohesin ATPase active sites.
Substitution of S83 to alanine (S83A) inhibited DI cohesion, while substitution of S83 to aspartic acid (S83D) to mimic phosphorylation generated cohesion during G2/M, even in absence of a DSB or Chk1.
The analogous substitution in the D-loop of the Smc1 ATPase active site, smc3-D1161E, nonlynly fails to act as a cohesion activator but also is unable to support viability, fails to load cohesin to DNA, and cannot generate cohesion.
The process of sister chromatid cohesion involves the binding of cohesin to chromosomes prior to DNA synthesis, and the subsequent tethering of sister chromatids by the DNA-bound cohesin to generate cohesion during S phase (Onn et al., 2008).
The mutant analysis described here reveals the unexpected molecular complexity of meiotic SCC and suggests that the linkages that tether sisters in the germline are not formed synchronously during DNA replication, as occurs during mitosis, but rather through the sequential action of proteins that bind to chromosomes at temporally distinct times and generate cohesion by different mechanisms.
Quorum responses are a ubiquitous feature of consensus decision-making and previous work proved their importance in generating aggregation, cohesion and decision accuracy [60] [61].
Dover inferred that … … if in addition, future studies confirm that many non-genic families affect DNA transcription and transcript processing, or chromatin structure and chromosome behaviour, then the dual process of generating intrapopulation cohesion and interpopulation discontinuities would be of considerable evolutionary significance.
(p. 115) … if in addition, future studies confirm that many non-genic families affect DNA transcription and transcript processing, or chromatin structure and chromosome behaviour, then the dual process of generating intrapopulation cohesion and interpopulation discontinuities would be of considerable evolutionary significance.
