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To test the generality of the NEM procedure for generating bound conformations, the validation study with crystal structure AJUXUY was repeated with 21 additional small molecule complex crystal structures (Table 2).
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These results show that the NEM procedure can generate bound conformations in the absence of their host structures regardless of the number of local minimum conformations or the molecular complexity.
UCH-L1 belongs to the family of cysteine proteases and functions as a deubiquitinase which generates, binds and stabilizes ubiquitin monomers, and thus can replenish the cellular monoubiquitin pool.
However, rather than being severely mis-folded and hence unable to bind α1-adrenoceptor ligands, the mutant that we have generated binds antagonist ligands with 'wild-type' affinity and actually binds agonist ligands with higher affinity than the wild-type α1b-adrenoceptor.
It also demonstrates the generality of the NEM procedure for generating small molecule bound conformations.
Prior to γ-secretase cleavage, the ectodomain of APP is shedded by β-secretase or α-secretase [5], [6], generating membrane bound C-terminal fragments (CTFs) which are further processed by γ-secretase.
If IRBs are willing to accept a separate consent form, this may pave the way for a caBIG-wide form with common language that could be appended to studies generating caBIG bound data.
Paris Fashion Week for ready-to-wear runs through Wednesday, but whatever buzz that Dior's line generates is bound to be drowned out by the fashion world's collective gasp over John Galliano.
The CO2 generated was bound to KOH to prevent interference with the measurement.
In OSCs, photons are absorbed in the photoactive layer and generate excitons (bound electron hole pairs).
Each subsequent RSA key pair that the TPM generated is bound to this original SRK.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com