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The development of a draft genome sequence using SGS technologies generally involves three main steps: generating and assembling short sequence reads into longer DNA contigs, ordering contigs along chromosomes, and annotating protein-coding genes and other elements for the contigs [ 1, 2].
In recent years, new sequencing technologies have greatly improved our capacity of generating and assembling complex genomes as well as our ability of mapping and quantifying virtually all genes present in any given transcriptome with extreme precision [ 24, 25].
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Genomic contigs were generated and assembled by using the Newbler assembler software (Roche Diagnostics, Basel, Switzerland).
Genomic contigs were generated and assembled by using the Newbler assembler software (Additional file 2: Table S3).
To evaluate the improvement in assembly quality with mate-pairs information we generated and assembled datasets similar to simHC, simLC and simMC with paired-ended reads of insert length 2000 bases.
This diversity poses general questions for neural circuit analysis: how are these interneuron cell types generated and assembled into stereotyped local circuits and how do they differentially contribute to circuit operations that underlie cortical functions ranging from perception to cognition?
Genome-wide short-read sequences (Hi-Seq, 500-bp inserts) were generated and assembled for one non-echolocating (Eidolon helvum) and three echolocating bats (Rhinolophus ferrumequinum, Megaderma lyra and Pteronotus parnellii).
The cover letter was generated and assembled with the citation, brochure, photos and return envelope and inserted into a larger envelope that was run through the postage meter and put in the U.S. mail for delivery.
Briefly, from the previous study, 438,321 ESTs were generated and assembled into contigs and singletons [11].
Six Gbp of data were generated and assembled into unigenes.
The 454 reads were generated and assembled by Roche.
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