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It is clear that our structure is just a first step to understanding Parkin activation, and future work should focus on generating active protein, and activated structures of Parkin.
We conclude that a significant part of myosin activity is utilized for generating active torques.
ERAP1 also enhances phagocytic activity of human macrophages through generating active peptides [ 38].
The precursor protein was activated through specific cleavage at the engineered sites by thrombin, generating active caspase-3.
The precursor protein was easily purified and activated through specific cleavage at the engineered sites by thrombin, generating active caspase-3 in high yields.
Silica-based sol-gel enzyme immobilization aided by polyamines has been used for generating active and stable heterogeneous biocatalysts.
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Procaspase-3 was cleaved by activated caspase-9 to generate active p20/p17 fragments (Fig. 1B).
We have reported that Leptospira are able to bind plasminogen (PLG), to generate active plasmin in the presence of activator, and to degrade purified extracellular matrix fibronectin.
Death-inducing signaling complex assembly confers autocatalytic activity onto pro-caspase-8 and generates active caspase-8, thereby initiating the caspase cascade.
It was reported that calpain II can cleave procaspase 12 to generate active caspase 12, which is capable of directly activating caspase 3 that leads to apoptosis [ 21].
Plasminogen-binding proteins were able to generate active plasmin in the presence of urokinase-type PLG activator, as measured by the cleavage of plasmin-specific chromogenic substrate.
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