Sentence examples for generating a strain from inspiring English sources

With our newly developed Di-CRISPR platform, we were able to attain highly efficient and markerless integration of large biochemical pathways and achieve an unprecedented 18-copy genomic integration of a 24 kb combined xylose utilization and (R,R -2,3-butanediol (BDO) pR,R -2,3-butanedioln a single step, thus geneR,R -2,3-butanediolt was aBDO to productionO directly from xylose.

Detection of the endogenous cyclin C protein was attempted by generating a strain in which an epitope tag was inserted into the endogenous gene.

Using recombination, we succeeded in generating a strain that was homozygous for a part of the introgressed region on chromosome 1 (location: 54.8 60.6 cM).

The third diploid was made by mating the trk1 Δ0 in the K12 background to BY, generating a strain that only differed from the control in that the only functional allele of TRK1 only was the BY allele.

The aim of using E. coli cells to economically produce therapeutically relevant, glycosylated human protein therapeutics, requires generating a strain which harbours the highest efficiency of post-translationally modified target proteins.

Introduction of four genes from C. acetobutylicum (ctfA, ctfB, adc and thiolase (thl)) into E. coli generated a strain capable of producing acetone (Bermejo et al.1998).1998

Among the 18 stopped projects were at least five working on adapting the MERS virus to mice in order to generate a strain that sickens the animals.

Therefore, we generated a strain co-expressing the premnaspirodiene oxygenase of Hyoscyamus muticus (HPO) and the Arabidopsis thaliana cytochrome P450 reductase (CPR) that hydroxylated extracellularly added -valencene.

The results demonstrated that the thermal actuator was able to generate a strain of 5% and a stress of 1 MPa for an input electric energy of 25 J, i.e. a strain comparable to human muscles mechanical properties.

In order to overcome this problem, we have generated a strain that is both hypersensitive to Hsp90 inhibitors as it lacks multiple drug resistance genes, and in which different heterologous and mutant Hsp90s can be expressed by plasmid exchange.

In order to establish this connection, we generated a strain of M. smegmatis, which is devoid of Dps2 gene and named this knockout strain as KK09.