In fact, Eph receptors are highly conserved and generating a site-specific antibody is extremely challenging.
There are some online tools that will automatically generate a site map for you such as XML-Sitemaps.
Thus, we generated a site-specific protease for synthetic approaches requiring in vivo generation of proteins or peptides with a specific N-terminal amino acid.
Cas9-guide RNA complexes recognize 20-base-pair sequences in DNA and generate a site-specific double-strand break, a robust activity harnessed for genome editing.
Cas9 nickase and a pair of sgRNAs that bind to the opposite strand of DNA generate a site-specific DSB and are used to improve the specificity of the genome editing.
Subsequent in situ ATRP of oligo ethylene glycol) methyl ether methacrylate (OEGMA) from the c-GFP-Br generated a site-specific (C-terminal) and stoichiometric (1 1) c-GFP-POEGMA conjugate with almost quantitative conversion and highly retained activity.
This recommendation was based on root-growth (Watson and O'Loughlin 1990) and canopy-development data (Grace et al. 1987) used to generate a site-occupancy model.
Data from the needs assessment, plus information on receipt of services along the Cascade from JJ and BH agency youth record systems were used by RCs to generate a site-specific, written feedback report.
We generated a site-specific point mutant in which the invariant core tyrosine residue 135 was substituted with a phenylalanine (Y135F), and expressed this from an episomal plasmid in a strain lacking endogenous RUP1.
