These long-term effects of TGFβ1 in HUTEC involve genes that are highly interconnected, thereby generating a scale-free network that we named the "TGFβ1 interactome", whose hubs represent proteins that may have a crucial role for HUTEC in response to TGFβ1.
In order to generate a scale of signal-to-noise ratio, we vary the spot intensity from 20 to 200 intensity levels.
This approach did not require any specialised training or extra clinical assessments, and was able to generate a scale with robust construct and predictive validity from data recorded as part of routine clinical care.
Three scaling assumptions should be satisfied for scale scores to be generated using the proposed item groups, and Likert's method of summated ratings [ 14, 15]. 1. Items in each scale should measure a common underlying construct otherwise it is not appropriate to combine them to generate a scale score [ 16].
We also investigated the effect of each somatic condition on spousal distress separately.> The Nord-Trøndelag Health Study 1 (HUNT 1, see " Sample" section) did not include an established measure of mental health, therefore we had to generate a scale from available items pertaining to mental health.
14 A composite indicator of socioeconomic deprivation for each postcode sector was derived using the Index of Multiple Deprivation (IMD) from 2007, which uses census-derived indicators of income, education, employment, environment, health and housing at small-area level to generate a scale from 0 (least deprived) to 80 (most deprived).
In each simulation, we generated a scale-free undirected and no-self-loop network with 500 nodes.
Our results are consistent with physical laws in which increased cell size is expected to result in relative reduction of plasma membrane production as volume grows faster than surface area (r versus r, where r is radius) and generates a scaling problem for a growing cell.
These values were then summed to generate a score (scale 0 7) that assessed self-reported frequency with which participants performed specific protective behaviours recommended by public health authorities.
Every item was scored with a Likert scale ranging from 1 to 7, generating a total scale range from 13-91, a high score representing a strong SOC.
Through factor analyses on the adapted items, four scales were generated: a Gender Scale (GS), a Girl Typicality Scale (GTS), a Boy Typicality Scale (BTS), and a Cross-Gender Scale (CGS), together constituting the 32-item CPBAQ.
