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Four amino acid residues of the third hypervariable loop (CDR3) of both VH and VL were combinatorially mutated, generating a repertoire of approximately 5 × 107 independent scFvs, cloned in a phagemid vector.
B cells are responsible for generating a repertoire of antibodies of virtually unlimited diversity in order to confront the antigenic universe.
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By combining 4 of these building blocks we generated a repertoire of 20,736 (124) sequences of 28 nucleotides.
Moreover, the ability to generate a repertoire of variants with distinct behavioral and physiological traits within clonal (asexual) reproduction plays an important role in epigenetic studies [ 3, 4].
It has been proposed that somatic recombination in the variable LRR-units has the potential to generate a repertoire of 1014 different lymphocyte receptors.
Later, NE cells switch to a neurogenic phase, involving asymmetric divisions that generate a repertoire of neurons with different identities depending on their birth order (reviewed by Jacob et al., 2008; Okano and Temple, 2009).
Two new mouse models have provided insight into how aberrations in T-cell development can generate a repertoire of autoreactive effector T cells that are necessary and sufficient to promote spontaneous inflammatory arthritis.
AND, OR and NOT logic gates were then layered in both parallel and serial arrangements to generate a repertoire of cellular Boolean operations that include NIMPLY, XOR, half adder and half subtractor logic operations.
Using a synthetic biology approach that is supported by computational modelling and rigorous characterisation, AND, OR and NOT biological logic gates were layered in both parallel and serial arrangements to generate a repertoire of Boolean operations that include NIMPLY, XOR, half adder and half subtractor logics in a single cell.
Using the methodology for synthesizing basis behaviors as a substrate for generating a large repertoire of higher-level group interactions, in this paper we describe how, given the substrate, greedy agents can learn social rules that benefit the group as a whole.
During differentiation, T cells undergo VDJ recombination generating a diverse repertoire of TCRs.
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