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In the above simulations, equal probability of generating a read from any step along an amplicon was assumed.
Given a set of reads and optional quality scores, Phrap computes overlaps and assembles the reads into contigs, generating a read multi-alignment, a contig sequence and sequence quality information.
We simulate this by generating a read that consists entirely of Ns with low quality scores, forcing it not to map.
Thus, We assume that the probability of generating a read from a specific transcript, t n, is proportional to the product of the relative frequency of the transcript,, and the length of the transcript: where, which is the expected length of a transcript given the PSG.
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The modMine group mitigates this somewhat by generating a read-only Chado database with submissions partitioned by PI rather than by individual submission, which they use to build the modMine query database.
Depending on which allele we consider as the ancestral allele, we can describe these SNPs as either introducing a premature stop codon (therefore producing a truncated protein) or as generating a read-through codon (therefore producing a longer product).
Simultaneously, sequence reads were mapped to known references to generate a read set for de novo assembly.
Generally, if the input MinION® read do not span entirely the repeated element, NaS workflow is not able to generate a read of the same length than the MinION® template.
Once the mixing proportions θ d are generated, for each transcript read to generate, we randomly select a source using the mixing proportions θ d, then randomly select a transcript from which to generate a read using the multinomial distribution specified by the expression profile of the chosen source.
"We're testing prototypes that can generate a reading once per second," said Brian Otis and Parviz, co-founders of the project which they started at the University of Washington, in a joint blog post.
It said the prototype could "generate a reading once per second".
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