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This method, given a set of pathways and normalized gene expression data, allows the transformation of the data into pathway enrichment scores (a measure of the state of each pathway), generating a pathway expression matrix.
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When using FET, we addressed the question of how to use the collection of p-values for a defined set of pathways to generate a pathway signature with the aim of comparing different microarray experiments.
ChlamyCyc results from a genome-scale metabolic pathway reconstruction to generate a pathway database for Chlamydomonas.
We generated a pathway list showing the 15 most significant pathways in each category (Additional file 5: Table S2).
In the case of the biomass reaction, it may not be computationally feasible to generate a pathway fragment collection that traverses all substrates simultaneously, but one may compute cut sets for individual biomass components through separate pathway fragment runs.
The functionally annotated reads obtained from MG-RAST were used to generate a pathway and KO (KEGG orthology) term count matrix to evaluate differential functional expression using edgeR (v. 3.4.2).
Clicking the link on a pathway name generates a pathway information page that contains detailed gene pathways, hyperlinks to the source pathway annotation database, a list of other genes that are assigned to the same pathway, and p-values for pathway assignments (B in Figure 3).
The ability to generate a pathway-level model depicting flux of precursors to the formation of a specific polysaccharide requires knowledge of compartmentalization and the component reactions of the pathway.
In order to represent the protein-coding genes with significant DM at the pathway level, the summed β-values of the TSS200 regions of DM protein-coding genes were used to generate a pathway-based deregulation score matrix (PDS matrix).
But sulfide is also an inhibitor of this enzyme and is necessary to be consumed to produce cysteine, which in turn, is used to detoxify cyanide, thus generating a cyclic pathway (Fig. 1).
However, this was not the case for Lxr, suggesting that growth on l-arabinose in the A. nidulans Δ araR/Δ xlnR strain is not likely caused by isoenzymes generating a similar pathway as the normal PCP.
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