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We therefore aimed to generate a stable Tardbp deficiency model by TILLING (Targeted Induced Local Lesions in Genome).
We generated a 5 kb deficiency in tio (tio S21) (supplementary material Fig. S1) by imprecise excision of the P-element tio-Gal4 A4 (Tang and Sun, 2002).
Consistent with this, moderate suppression of Syx5 specifically in the adult thorax generated a typical copper-deficiency phenotype.
However, we must note that the RBL based self-referenced setups have to deal with some loss of the low-frequency components, which deficiency generates a diffraction-fringe-like noise on the reconstruction.
The software generates a calculation showing any funding deficiency they may have at retirement.
Therefore, Wang et al. have generated a mouse model of AC3 deficiency to test this hypothesis.
In order to investigate CrT deficiency, we generated a transgenic mouse with a portion of the CrT gene flanked by loxP sites.
By using an antisense knockdown strategy we generated a zebrafish model of parkin deficiency (knockdown efficiency between 50% and 60%) and found that the transient knockdown of parkin does not cause morphological or behavioral alterations.
To elucidate the molecular pathogenesis of TK2 deficiency, we generated a homozygous Tk2 H126N knock-in mutant (Tk2 −/− ) mouse that manifests a phenotype strikingly similar to the human infantile encephalomyopathy (Akman et al, 2008).
In contrast, mild suppression generates a phenotype typical of copper-deficiency in viable, fertile flies and is exacerbated by co-suppression of the copper uptake gene Ctr1A.
Is there an eater mutant or are the authors using a set of deficiencies to generate a mutant?
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